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Moreover, the gravimetric method's inherent accuracy makes it a preferred choice for regulatory compliance. Many manufacturing processes are subject to stringent quality regulations, and having reliable methods for determining material composition is fundamental to meeting these standards. Accurate gravimetric analysis assists manufacturers in minimizing waste, optimizing formulations, and ensuring that end products are both effective and safe.
The EU expert panel did not identify an immediate health concern linked to TiO2 when used as a food additive. However, due mainly to uncertainties concerning the safety of TiO2 nanoparticles, the panel concluded that TiO2 as a food additive (E171) could no longer be considered safe.
The vitaminC@P25TiO2NPs, on the other hand, were obtained through an optimized method based on Mallakpour et al. [27]. Initially, 0.02 g of P25TiO2NPs were dispersed in 1 mL of ultrapure water and stirred in a Vortex. Next, 100 μL of HCl (0.01 M) were added (pH 2) to 100 uL of P25TiO2NPs to avoid gel formation. Then, 100 μL of vitamin C dissolved in ultra-pure water (5.0 × 10−3 M) solution were added to the mixture and was ultrasonicated for 30 min. Finally, vitamin C was added in excess to gain a beige-orange color suspension, and the ultrasonication continued for another 30 min. The pellet obtained after centrifuging the suspension for 10 min at 4500 rpm was resuspended in ultrapure water, centrifuged again, and then lyophilized.
In general, nanoparticles have been shown to accumulate in the body, particularly in organs in the gastrointestinal tract, along with the liver, spleen, and capillaries of the lungs.