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In conclusion, food-safe titanium dioxide has played a significant role in enhancing the visual appeal of our food, but its use is continually being reassessed in light of new scientific evidence. Regulatory bodies worldwide are vigilant in ensuring its safety, and the industry is adapting to meet changing standards and consumer demands. As we move forward, it is crucial to strike a balance between innovation and safety, ensuring that the food we consume is not just visually appealing but also free from potential harm.

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No acute effects of nano-sized TiO2 were observed in Danio rerio (zebrafish) embryos. Exposure of rainbow trout to TiO2 NPs triggered lipid peroxidation, influence on the respiratory tract, disturbance in the metabolism of Cu and Zn, induction of intestinal erosion  and accumulation in kidney tissue. Linhua et al. exposed juvenile carp to 100 and 200 mg/ml of particles and TiO2 observed no mortality. However, the fish suffered from oxidative stress and pathological changes in gill and liver. In the infaunal species Arenicola marina, exposure to TiO2 NPs in sediment caused sub-lethal effects including decrease in casting rate and increase in cellular and DNA damage. Aggregated particles were visible in the lumen of the gut, but no uptake through the gut or the skin was observed.

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