lithopone is a mixture of

As awareness of the environmental consequences associated with industrial activities heightened, TiO2 industry factories began to adopt cleaner production technologies. Innovations such as the chloride process offered not only improved yields but also reduced energy consumption and waste generation. Moreover, the introduction of advanced filtration systems and waste treatment protocols significantly mitigated the environmental impact of TiO2 manufacturing.

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In a review published in 2022 in the journal Archives of Toxicologyresearchers found that the ingestion of E171 is a “a definite health risk for consumers and their progeny.” After reviewing dozens of in vivoex vivo and in vitro studies on the toxicity of E171, the researchers wrote that two facts must be noted: “First, reprotoxicity studies show that animals of both sexes are impacted by the toxicity of these nanoparticles, underlining the importance of conducting in vivo studies using both male and female animals. Second, human exposure begins in utero via maternal-fetal transfer and continues after birth by breastfeeding. Children are then chronically re-exposed due to their food preferences. To be relevant to the human in vivo situation, experimental studies should therefore consider nanoparticle exposure with respect to the age or life period of the studied population.”

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The vitaminC@P25TiO2NPs, on the other hand, were obtained through an optimized method based on Mallakpour et al. [27]. Initially, 0.02 g of P25TiO2NPs were dispersed in 1 mL of ultrapure water and stirred in a Vortex. Next, 100 μL of HCl (0.01 M) were added (pH 2) to 100 uL of P25TiO2NPs to avoid gel formation. Then, 100 μL of vitamin C dissolved in ultra-pure water (5.0 × 10−3 M) solution were added to the mixture and was ultrasonicated for 30 min. Finally, vitamin C was added in excess to gain a beige-orange color suspension, and the ultrasonication continued for another 30 min. The pellet obtained after centrifuging the suspension for 10 min at 4500 rpm was resuspended in ultrapure water, centrifuged again, and then lyophilized.

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