This constant high rate of ROS production leads rapidly to extreme macromolecular oxidation, here it is observed in the AOPP and MDA detected after 3 h in samples treated with bare P25TiO2NPs (Fig. 6, Fig. 7). Macromolecular oxidation includes, among others, both protein and lipid oxidation. The ROS causes protein oxidation by direct reaction or indirect reactions with secondary by-products of oxidative stress. Protein fragmentation or cross-linkages could be produced after the oxidation of amino acid side chains and protein backbones. These and later dityrosine-containing protein products formed during excessive production of oxidants are known as advanced oxidation protein products (AOPP). They absorb at 340 nm and are used to estimate the damage to structural cell amino acids. Lipid oxidation is detected by the conjugation of oxidized polyunsaturated lipids with thiobarbituric acid, forming a molecule that absorbs light at 532 nm. Polyunsaturated lipids are oxidized as a result of a free-radical-mediated chain of reactions. The most exposed targets are usually membrane lipids. The macromolecular damage could represent a deadly danger if it is too extensive, and this might be the case. Moreover, it could be observed that cellular damage continues further and becomes irrevocable after 6 h and MDA could not be detected. This may be due to the fact that the lipids were completely degraded and cells were no longer viable. Lipids from the cell membrane are the most prone to oxidation. In fact, lipid peroxidation biomarkers are used to screen the oxidative body balance [51]. At the same time, AOPP values are up to 30 times higher for bare nanoparticles in comparison to the functionalized ones.
In addition to the toxic effects of TiO2 NPs, discussed in previous chapters, these NPs have been also shown to promote photosynthesis and nitrogen metabolism, resulting in the enhanced growth of spinach. It increases the absorption of light and accelerates the transfer and transformation of the light energy. It was also found that treatment with nano-sized TiO2 significantly increased the level of antioxidant enzymes, and decreased the ROS accumulation and malonyldialdehyde content in spinach chloroplasts under visible and UV irradiation. TiO2 NPs also increased the superoxide dismutase activity of germinating soybean, enhanced its antioxidant ability, and promoted seed germination and seedling growth.
Moreover, the use of wholesale titanium dioxide anatase TIO2 in paint formulations contributes to energy efficiency during the production process
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Molded Fiberglass Grating
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molded frp gratings are manufactured in a one piece construction which results in a bi-directionally strong product. This allows openings for piping or cables in the grating of up to 10” without any additional support required (may vary depending on grating depth and location of opening). During the molded manufacturing process, the continuous fiberglass rovings are completely covered in a high percentage of resin, 65% by volume. With this high resin content, molded gratings are the most chemically resistant gratings offered and are often used in caustic chemical environments without hesitation.