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Moreover, with increasing concerns over sustainability, TIO2 pigment manufacturers are under pressure to develop more eco-friendly production methods. This includes recycling waste streams, using renewable energy sources, and reducing the carbon footprint associated with the extraction and processing of titanium ore This includes recycling waste streams, using renewable energy sources, and reducing the carbon footprint associated with the extraction and processing of titanium ore This includes recycling waste streams, using renewable energy sources, and reducing the carbon footprint associated with the extraction and processing of titanium ore This includes recycling waste streams, using renewable energy sources, and reducing the carbon footprint associated with the extraction and processing of titanium oretio2 pigment manufacturers. Some companies have even turned to synthetic biology to produce TIO2 pigments through microorganisms, aiming to create a bio-based alternative to traditional mining and chemical synthesis.

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Macromolecular oxidation was detected in proteins by the colorimetric measurement of Advanced Oxidation Protein Products (AOPP) and in lipids by the colorimetric quantification of malondialdehyde (MDA). Standard curves were run with chloramine-T and 1,1,3,3 tetraethoxypropane (TEP) for AOPP and MDA methods, respectively [29][30][31]. Values were normalized to initial protein content in samples, measured with Bradford reagent [32]. The standard deviation of at least six measures was calculated and p-value < 0.05 were considered significant.

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Titanium alloy is widely used as a biomaterial due to its superior biocompatibility, mechanical properties close to human bones, and enhanced corrosion resistance. These properties have made the alloys suitable for use in a wide spectrum of biomedical applications including artificial bones, artificial joints, dental roots, and medical devices. The excellent performance of titanium alloy is mainly due to the oxide film as shown in Figure 1 [1]. The functional composition of the oxide film is mainly titanium dioxide (TiO2). Titanium dioxide has good biocompatibility, stable chemical property, and low solubility in water, which prevents substrate metal ions from dissolution. Furthermore, it also improves the wear and fatigue resistance of implants in the human body.

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