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Testing samples were made mixing 100 uL of TiO2NPs suspensions (0.2 mg/mL and 0.02 mg/mL) and vitamins@P25TiO2NPs (0.2 mg/mL and 0.02 mg/mL) with 100 μL ATCC 29,213 methicillin-sensitive Staphylococcus aureus (MSSA) (107 in PBS, pH 7). Controls were made replacing nanoparticles with the same volume of PBS. The concentrations of nanoparticle suspensions were chosen based on the FDA approved maximal and the minimal amount usually found in sunscreens, which are 20% and 2% (this is equivalent to 0.2 mg/mL and 0.02 mg/mL for nanoparticles suspensions). The cream concentration, on the other hand, was an intermediate value of 10%.

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Furthermore, TiO2 is a versatile and environmentally friendly pigment that is widely used in the paper industry. It is non-toxic, biologically inert, and poses no harm to human health or the environment. As sustainability and eco-friendliness become increasingly important considerations for consumers, paper suppliers are turning to TiO2 as a safe and responsible choice for enhancing the quality of their products. TiO2 is also recyclable, making it a sustainable option for paper manufacturers looking to reduce their environmental footprint.

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