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Mexican researchers sought to evaluate the effects of E171 across a span of conditions in mice, including its influence on behavior, along with the effects on the colon and liver. The research, published in 2020 in the journal Food and Chemical Toxicology, showed that E171 promoted anxiety and induced adenomas, or noncancerous tumors, in the colon. They also found that E171 heightened goblet cells hypertrophy and hyperplasia, which is typically seen in asthma patients and triggered by smoking or external pollutants and toxins. They also noted mucins overexpression in the mice, which can be linked to cancer cell formation. 

According to a 2011 study published in Radiology and Oncology, titanium dioxide may lead to some adverse effects in the body, including oxidative stress, inflammation, and changes in cell signaling pathways. Additionally, pure titanium dioxide can be notably harmful when inhaled.

Genotoxicity and cytotoxicity