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2025-08-15 04:02
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The Scientific Committee on Consumer Safety issued an opinion of the safety of titanium dioxide in food, stating that it should no longer be considered as safe when used as a food additive.
The cytotoxic effect was tested through the colorimetric assay employing 3′-[1-[(phenylamino) -carbonyl]−3,4-tetrazolium]-bis(4‑methoxy-6-nitro) benzene-sulfonic acid hydrate (XTT) by reading the absorbance at 490 nm after 3 h of incubation post treatment [28]. The absorbance is proportional to the metabolic rate of viable (live) cells.