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The morphology of vitaminB2@P25TiO2NPs is coherent with the description of Degussa P25 typical population. Size distribution histograms were made from manual measures of the nanoparticles observed in SEM micrographs using ImageJ®. This data showed that more than 70% is anatase (between 20 and 60 nm) with a minor amount of rutile characteristic bars (between 80 and 100 nm) and a small amount of amorphous phase (<40 nm) [36]. Further analysis of the same sample areas with an EDS probe demonstrated the presence of organic material composed of C and O (Fig. 2). This material was found homogeneously distributed on the surface of the different shapes of P25TiO2NP, not in the background, indicating a specific interaction that could be attributed to the functionalization of the P25TiO2NPs with vitamin B2.

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The manufacturing process of titanium dioxide typically involves mining ilmenite, rutile, or anatase from natural sources, followed by conversion into TiO2 through various chemical processes. The resulting TiO2 can then be further processed to achieve different particle sizes and coatings that optimize its performance in specific applications. For instance, TiO2 used in sunscreens is often coated to enhance its effectiveness in blocking UV rays without causing skin irritation.

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