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Thermogravimetric analysis (TGA) was conducted in a sample of vitaminB2@P25TiO2NPs using a TA-THA Q5000 equipment. Temperature ramp rate: 10 °C/min, maximum temperature: 1000 °C, under air. Part of the same sample was mounted on conductive copper tape grids and observed through a Carl Zeiss Sigma scanning electron microscope (SEM) with an EDS probe, at the “Laboratorio de Microscopía y Análisis por Rayos X” (LAMARX) of National University of Córdoba (Argentina).

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We've measured many different immunological markers, both in the mucosa of the intestine and the lymphoid organs part of the intestine and we didn't see any inflammation there or changes in white blood cell number, Kaminski said. They also looked at the spleen where titanium dioxide was taken up into the bloodstream. You would expect [titanium dioxide] would be filtered out in the spleen and maybe induce inflammation there, but we did not see that, Kaminski added.

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