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In conclusion, lithopone pigments, as manufactured by dedicated producers worldwide, are integral to numerous industries. Their production involves a complex yet precise process, and the manufacturers' continuous efforts to enhance product quality and sustainability ensure the ongoing relevance of lithopone pigments in the global market. With the increasing demand for efficient and environmentally friendly materials, the future of lithopone pigments as a key pigment choice appears promising.

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Should a grade of lithopone be desired higher than the standard grade and another by-productas, for example, a forty-five*per= cent. grade of lithopone and barium chlorid the following process may be pi'acticed,\vhich, however, is quite analogous to the one already 10o described. Aqueous solutions of the ingredients are prepared as before, in these proportions: zinc sulfate, one hundred and sixty-one pounds; zinc chlorid, one hundred and thirty-six pounds, and barium sulfid, three hundred and thirty-eight pounds. Upon mixing these several solutions the lithopone will at once be precipitated in accordance with the following reaction:

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Hemolysis was studied on suspensions of P25TiO2NPs (0.2 mg/mL and 0.02 mg/mL), vitaminB2@P25TiO2NPs (0.2 mg/mL and 0.02 mg/mL) and vitamin B2 (0.2 mg/mL and 0.02 mg/mL) were prepared and mixed with 500 μL of anticoagulated blood (donated by Laboratorio de Hemoderivados, UNC) in a rate of 1/10. A solution of NaCl 10% was used as the positive control and PBS as the negative control. Then, the samples were irradiated using the LED described above for 3 and 6 h to simulate the light penetration into the skin. Also, a set of samples was kept in the dark as control. Finally, the samples were centrifuged and the absorbance at 540 nm was measured in the supernatants. The experiment was reproduced twice; the standard deviation was calculated and p-value < 0.05 were considered significant.

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