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The integrity of surface skin cells was evaluated with and without solar simulated irradiation. The integrity of the stratum corneum was significantly lower in individuals treated with P25TiO2NPs under the light in comparison to the ones that received the functionalized nanoparticles. Cell membrane suffering is evident (Fig. 9), and it is in accordance with the ROS levels and macromolecule oxidation found in vitro for the irradiated P25TiO2NPs. Disruption of the superficial skin layer was observed in all animals treated with no functionalized nanoparticles, under irradiation. This data expands the findings by the group of Professors Fubini and Fenoglio, who showed that P25TiO2NPs could impact the lipid structure at the top few microns of the stratum corneum [55]. Control skin under irradiation and without any topic formulation did not show changes in cell structure.

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It should also be considered that due to the low pH in the stomach, the increased dissolution of the TiO2 particles may increase its bioavailability and may facilitate the entry of titanium ions into the blood circulation. Despite the relatively large consumption of TiO2 as a food additive, no studies on the effect of pH on its absorption and bioavailability have been found in the literature. This can be attributed to a general belief that TiO2is completely insoluble. However, this is not completely true, as TiO2 particles show a certain degree of solubility.

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Anatase, on the other hand, is a titanium dioxide form that exhibits higher photocatalytic activity and lower photocorrosion compared to rutile. It is commonly used in sunscreens, cosmetics, and water treatment due to its ability to absorb ultraviolet light and protect skin from harmful UV radiation. Anatase titanium dioxide is typically produced by the chloride process, which involves the chlorination of titanium ore to produce titanium tetrachloride Anatase titanium dioxide is typically produced by the chloride process, which involves the chlorination of titanium ore to produce titanium tetrachloride Anatase titanium dioxide is typically produced by the chloride process, which involves the chlorination of titanium ore to produce titanium tetrachloride Anatase titanium dioxide is typically produced by the chloride process, which involves the chlorination of titanium ore to produce titanium tetrachloriderutile and anatase titanium dioxide factory. The resulting gas is then reacted with oxygen to produce anatase titanium dioxide particles, which are collected and processed into the final product.

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