In a study published in the journal Toxicology, researchers examined the effects of exposing human colon cancer cell line (HTC116) titanium dioxide food additives in vitro. “In the absence of cytotoxicity, E171 was accumulated in the cells after 24 hours of exposure, increasing granularity and reactive oxygen species, inducing alterations in the molecular pattern of nucleic acids and lipids, and causing nuclei enlargement, DNA damage and tubulin depolymerization,” the scientists wrote. Researchers removed the additive from the culture, then examined the results 48 hours later. They found, “The removal of E171 was unable to revert the alterations found after 24 h of exposure in colon cells. In conclusion, exposure to E171 causes alterations that cannot be reverted after 48 h if E171 is removed from colon cells.”

In recent years, environmental concerns have shaped the way lithopone is produced and used. Suppliers are now more attentive to the sustainability aspect of their operations, ensuring that lithopone is sourced from eco-friendly processes. Many suppliers have adopted responsible mining practices and have invested in technologies that reduce waste and emissions during production. This shift not only appeals to environmentally-conscious consumers but also helps manufacturers comply with stringent regulations regarding product safety and environmental impact.