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In a study published in the journal Toxicology, researchers examined the effects of exposing human colon cancer cell line (HTC116) titanium dioxide food additives in vitro. “In the absence of cytotoxicity, E171 was accumulated in the cells after 24 hours of exposure, increasing granularity and reactive oxygen species, inducing alterations in the molecular pattern of nucleic acids and lipids, and causing nuclei enlargement, DNA damage and tubulin depolymerization,” the scientists wrote. Researchers removed the additive from the culture, then examined the results 48 hours later. They found, “The removal of E171 was unable to revert the alterations found after 24 h of exposure in colon cells. In conclusion, exposure to E171 causes alterations that cannot be reverted after 48 h if E171 is removed from colon cells.”

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To address this challenge, many manufacturers are turning to biotechnology as a viable solution. Bioprocesses, such as the use of microorganisms or enzymes, offer a more sustainable alternative to traditional chemical methods. These processes can significantly reduce the amount of energy and chemicals required, while also generating fewer byproducts These processes can significantly reduce the amount of energy and chemicals required, while also generating fewer byproducts These processes can significantly reduce the amount of energy and chemicals required, while also generating fewer byproducts These processes can significantly reduce the amount of energy and chemicals required, while also generating fewer byproductsr 5566 titanium dioxide factories.

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The conventional surface treatment methods of titanium alloy include glow discharge plasma deposition, oxygen ion implantation, hydrogen peroxide treatment, thermal oxidation, sol-gel method, anodic oxidation, microarc oxidation, laser alloying, and pulsed laser deposition. These methods have different characteristics and are applied in different fields. Glow discharge plasma deposition can get a clean surface, and the thickness of the oxide film obtained is 2 nm to 150 nm [28]. The oxide film obtained from oxygen ion implantation is thicker, about several microns [914]. Hydrogen peroxide treatment of titanium alloy surface is a process of chemical dissolution and oxidation [1516]. The dense part of the oxide film is less than 5 nm [1721]. The oxide film generated from the thermal oxidation method has a porous structure, and its thickness is commonly about 10-20 μm [2225]. The oxide film from the sol-gel method is rich in Ti-OH, a composition that could induce apatite nucleation and improve the combining of implants and bone. It has a thickness of less than 10 μm [2628]. Applied with the anodic oxidation method, the surface can generate a porous oxide film of 10 μm to 20 μm thickness [2931]. Similarly, the oxide film generated from the microarc oxidation method is also porous and has a thickness of 10 μm to 20 μm [3233].

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