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In a small study published in the European Journal of Nutrition in 2020, researchers examined the effects of several food additives, including titanium dioxide, along with artificial sweeteners and cleaning products by testing the fecal samples of 13 people. Titanium dioxide was among the samples that “induced significant shifts in microbiome community structure.”  The growth of the bacterium species belonging to C. leptum, which has been shown to decrease in patients with inflammatory bowel disease, “significantly decreased in the presence of … titanium dioxide” among other additives and sweeteners tested.

In terms of application, meticulous preparation and attention to detail yield the best results. For paint grinders, maintaining a ratio of 12 pounds of refined linseed oil to 88 pounds of lithopone pigment will provide optimal workability. A salient factor that should be heeded is the state of the lithopone before mixing with oil; the material must be sufficiently dry. Only then will it integrate seamlessly with the oil, ensuring that the resultant mixture possesses the desired consistency and properties.